The effects of high temperature level on square Bt protein concentration of Bt cotton

作者：Jun Wang, Yuan Chen, Meng-hao YAO, Yuan Li, Yujin Wen, Chen Yuan, Xiang Zhang, De-hua CHEN · 发表于：Journal of Integrative Agriculture · 年份：2015 · DOI：10.1016/s2095-3119(15)61049-8 · 被引用次数：45 · 研究领域：Insect Resistance and Genetics、Insect and Pesticide Research、Genetically Modified Organisms Research

Higher boll worm survival rates were detected after high temperature presented during square period in Bt cotton. The objective of this study was to investigate the effects of high temperature level on the Bt efficacy of two different types of Bt cotton cultivars at squaring stage. During the 2011 to 2013 cotton growth seasons, high temperature treatments ranged from 34 to 44°C in climate chambers, and field experiments under high temperature weather with various temperature levels were conducted to investigate the effects of the high temperature level on square Bt protein concentration and nitrogen metabolism. The climate chamber experiments showed that the square insecticidal protein contents reduced after 24 h elevated temperature treatments for both cultivars, whereas significant declines of the square insecticidal protein contents were detected at temperature >38°C, and only slightly numerical reductions were observed when temperature below 38°C. Similar high temperature responses were also observed at the two field experimental sites in 2013. Correspondingly, high temperature below 38°C seems have little effect on the square amino acid concentrations, soluble protein contents, glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminase (GOT) activities as well as protease and peptidase activities; however, when the temperature was above 38°C, reduced soluble protein contents, enhanced amino acid concentrations, decreased GPT and GOT activities, bolstered pr...