Identification of two proteins encoded by the Saccharomyces cerevisiae GAL4 gene.

作者：Allen S. Laughon, Robert M. Driscoll, Norma M. Wills, R F Gesteland · 发表于：Molecular and Cellular Biology · 年份：1984 · DOI：10.1128/mcb.4.2.268 · 被引用次数：44 · 研究领域：Plant biochemistry and biosynthesis、Microbial Metabolic Engineering and Bioproduction、Biofuel production and bioconversion

We placed the Saccharomyces cerevisiae GAL4 gene under control of the galactose regulatory system by fusing it to the S. cerevisiae GAL1 promoter. After induction with galactose, GAL4 is now transcribed at about 1,000-fold higher levels than in wild-type S. cerevisiae. This regulated high-level expression has enabled us to tentatively identify two GAL4-encoded proteins.