Metabolic compartmentation of plastid prenyllipid biosynthesis

作者：Yves Keller, Florence Bouvier, Alain d’Harlingue, Bilal Camara · 发表于：European Journal of Biochemistry · 年份：1998 · DOI：10.1046/j.1432-1327.1998.2510413.x · 被引用次数：185 · 研究领域：Photosynthetic Processes and Mechanisms、Lipid metabolism and biosynthesis、Plant biochemistry and biosynthesis

The addition of phytyl side chain to chlorophylls, tocopherols and phylloquinone is prerequisite to their integration into plastid membranes. We have cloned a cDNA encoding a pre-geranylgeranyl reductase from Arabidopsis thaliana. The deduced primary structure predicts a mature size with a molecular mass of 47 kDa and displays a characteristic dinucleotide binding domain. Geranylgeranyl reductase expressed in Escherichia coli sequentially catalyzes the reduction of geranylgeranyl-chlorophyll a into phytyl-chlorophyll a as well as the reduction of free geranylgeranyl diphosphate into phytyl diphosphate. Due to its multifunctionality and weak hydrophobicity, we suggest that in plastid the same geranylgeranyl reductase is recruited into the chlorophyll, the tocopherol and the phylloquinone pathways. The geranylgeranyl reductase gene is up-regulated during etioplast to chloroplast and chloroplast to chromoplast development.