Mechanistically novel iron(III) transport system in Serratia marcescens

作者：L Zimmermann, Annemarie Angerer, V Braun · 发表于：Journal of Bacteriology · 年份：1989 · DOI：10.1128/jb.171.1.238-243.1989 · 被引用次数：69 · 研究领域：Bacterial Genetics and Biotechnology、Enzyme Structure and Function、Porphyrin Metabolism and Disorders

A novel iron(III) transport system of Serratia marcescens, named SFU, was cloned and characterized in Escherichia coli. Iron acquisition by this system differed from that by E. coli and related organisms. No siderophore production and no receptor protein related to the SFU system could be detected. In addition, iron uptake was independent of the TonB and ExbB functions. On the cloned 4.8-kilobase sfu fragment, two loci encoding a 36-kilodalton (kDa) protein and three proteins with molecular masses of 40, 38, and 34 kDa were identified; the 40-kDa protein represents a precursor form. Furthermore, chromosomally encoded functions of E. coli were required for the uptake of iron by this system.