Rapid biochemical tests for characterization of the Mycoplasmatales

作者：Janet M. Bradbury · 发表于：Journal of Clinical Microbiology · 年份：1977 · DOI：10.1128/jcm.5.5.531-534.1977 · 被引用次数：83 · 研究领域：Microbial infections and disease research、Aquaculture disease management and microbiota、Bacteriophages and microbial interactions

Methods are described for the rapid detection of beta-D-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. These methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. Results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the conventional tests took several days.