Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms

作者：E D Kopczynski, Mary M. Bateson, David M. Ward · 发表于：Applied and Environmental Microbiology · 年份：1994 · DOI：10.1128/aem.60.2.746-748.1994 · 被引用次数：217 · 研究领域：Microbial Community Ecology and Physiology、Environmental DNA in Biodiversity Studies、Genomics and Phylogenetic Studies

When PCR was used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains.