Evidence from UV transcription mapping that late adenovirus type 2 mRNA is derived from a large precursor molecule

作者：Seth Goldberg, Joseph R. Nevins, James E. Darnell Jr. · 发表于：Journal of Virology · 年份：1978 · DOI：10.1128/jvi.25.3.806-810.1978 · 被引用次数：38 · 研究领域：Animal Genetics and Reproduction、RNA and protein synthesis mechanisms、Virus-based gene therapy research

The UV inactivation of labeling of various late adenovirus type 2 mRNA's is as great or greater than the inactivation of labeling of the same sequences that are present in a large primary RNA transcript in the cell nucleus. Thus, the large nuclear adenovirus type 2-specific RNA appears to be the obligatory precursor of cytoplasmic mRNA. The majority of the mRNA's derived from the large precursor apparently require the synthesis of the whole nuclear molecule. A UV-sensitive target necessary for proper processing of all the mRNA's may exist near the end of the transcription unit.