The N,O-Diacetylmuramidase of Chalaropsis Species

作者：John H. Hash, M. V. Rothlauf · 发表于：Journal of Biological Chemistry · 年份：1967 · DOI：10.1016/s0021-9258(18)99397-x · 被引用次数：104 · 研究领域：Enzyme Structure and Function、Genomics and Phylogenetic Studies、Antibiotic Resistance in Bacteria

Abstract The bacteriolytic enzyme from the fungus, Chalaropsis species, has been purified and crystallized in high yield. The needle-shaped crystals were approximately 10 µ long. The crystalline protein exhibited a single band in polyacrylamide gel electrophoresis at pH values of 9.3, 7.5, and 4.3. A single amino acid, threonine, was obtained by conventional NH2-terminal analysis. The specificity of the enzyme was confirmed to be an N,O-diacetylmuramidase.