Molecular cloning and characterization of the pgm gene encoding phosphoglucomutase of Escherichia coli

作者：Min Lu, Nancy E. Kleckner · 发表于：Journal of Bacteriology · 年份：1994 · DOI：10.1128/jb.176.18.5847-5851.1994 · 被引用次数：128 · 研究领域：Enzyme Structure and Function、Amino Acid Enzymes and Metabolism、Bacterial Genetics and Biotechnology

We report here the identification and characterization of pgm, a gene in Escherichia coli that encodes the enzyme phosphoglucomutase, specifically required for the catalysis of the interconversion of glucose 1-phosphate and glucose 6-phosphate. The predicted amino acid sequence of the pgm gene is highly conserved in E. coli, Acetobacter xylinum, Saccharomyces cerevisiae, rabbits, and humans. pgm deletion mutant strains are deficient in phosphoglucomutase activity.