The Activation of Arginyl Transfer Ribonucleic Acid Synthetase by Transfer Ribonucleic Acid

作者：Alan H. Mehler, Samir Kumar Mitra · 发表于：Journal of Biological Chemistry · 年份：1967 · DOI：10.1016/s0021-9258(18)99386-5 · 被引用次数：113 · 研究领域：Biochemical and Molecular Research、Pneumocystis jirovecii pneumonia detection and treatment、Chemical Synthesis and Analysis

Abstract The arginine-activating enzyme of Escherichia coli requires transfer ribonucleic acid (tRNA) for catalysis of the ATP-pyrophosphate exchange reaction. Only the specific arginine-accepting tRNA is effective, and the terminal adenylic acid residue is essential for the reaction. A previously described isotope-trapping experiment led to the suggestion that an arginyl-tRNA ester was not formed during the pyrophosphate exchange. The specific tRNA has now been shown to be required for reaction of this enzyme system with hydroxylamine and for the enzymatic pyrophosphorolysis of arginyl adenylate.