The Outer Membrane of Gram-Negative Bacteria Inhibits Antibacterial Activity of Brochocin-C

作者：Yan Ming Gao, Marco J. van Belkum, Michael E. Stiles · 发表于：Applied and Environmental Microbiology · 年份：1999 · DOI：10.1128/aem.65.10.4329-4333.1999 · 被引用次数：175 · 研究领域：Probiotics and Fermented Foods、Antimicrobial Peptides and Activities、Bacterial Genetics and Biotechnology

Brochocin-C is a two-peptide bacteriocin produced by Brochothrix campestris ATCC 43754 that has a broad activity spectrum comparable to that of nisin. Brochocin-C has an inhibitory effect on EDTA-treated gram-negative bacteria, Salmonella enterica serovar Typhimurium lipopolysaccharide mutants, and spheroplasts of Typhimurium strains LT2 and SL3600. Brochocin-C treatment of cells and spheroplasts of strains of LT2 and SL3600 resulted in hydrolysis of ATP. The outer membrane of gram-negative bacteria protects the cytoplasmic membrane from the action of brochocin-C. It appears that brochocin-C is similar to nisin and possibly does not require a membrane receptor for its function; however, the difference in effect of the two bacteriocins on intracellular ATP indicates that they cause different pore sizes in the cytoplasmic membrane.