Isolation of a maltase structural gene from Saccharomyces carlsbergensis

作者：Howard J. Federoff, Joe D. Cohen, T. Ross Eccleshall, Richard B. Needleman, Barbara Buchferer, Joseph C. Giacalone, Julius Marmur · 发表于：Journal of Bacteriology · 年份：1982 · DOI：10.1128/jb.149.3.1064-1070.1982 · 被引用次数：71 · 研究领域：Enzyme Structure and Function、Fungal and yeast genetics research、Bacterial Genetics and Biotechnology

The maltase structural gene MAL6 of the yeast Saccharomyces carlsbergensis has been cloned by transformation of a maltose nonfermenting recipient strain with autonomously replicating chimeric recombinant plasmids. One recombinant plasmid, pMAL26, was shown by positive hybridization translation, as well as by Southern and Northern blot experiments, to carry the MAL6 structural gene.