Effects of elevated temperatures and drugs on the viability of L1210 leukemia cells.

作者：Beppino C. Giovanella, Wendy A. Lohman, Charles Heidelberger · 发表于：PubMed · 年份：1970 · 被引用次数：95 · 研究领域：thermodynamics and calorimetric analyses

Summary The lethal action of elevated temperatures on neoplastic cells has been determined quantitatively by means of an in vitro-in vivo system of L1210 leukemia cells. Temperatures from 37° to 40° have little effect on these cells. Prolonged exposure at 41° impairs their viability. Between 41° and 42° the lethal effect increases markedly; in Fischer medium, a 4-log kill is achieved at 42° in 3 hr. The exposure of L1210 leukemia cells to a temperature of 42° for a short time sensitizes them to the action of lower temperatures. A 3-hr exposure at 40° has a highly lethal effect when applied after an initial treatment at 42°. If the sequence of heating is reversed, no such effect is observed. Many drugs have been tested for combined therapy. l-erythro-α,β-Dihydroxybutyraldehyde has been found to act synergistically with heat. dl-Glyceraldehyde, l-phenylalanine mustard, actinomycin D, and sodium oxamate are also active in combination with heat. However, if toxicity to humans is considered, only dl-erythro-α,β-dihydroxybutyraldehyde, dl-glyceraldehyde, and phenylalanine mustard can be considered as suitable for clinical trial in combination with heat.