The Glucagon-sensitive Adenyl Cyclase System in Plasma Membranes of Rat Liver

作者：Lutz Birnbaumer, Stephen L. Pohl, Martin Rodbell · 发表于：Journal of Biological Chemistry · 年份：1971 · DOI：10.1016/s0021-9258(18)62387-7 · 被引用次数：167 · 研究领域：Drug Transport and Resistance Mechanisms、Ion Transport and Channel Regulation、Peroxisome Proliferator-Activated Receptors

Abstract Glucagon and fluoride ion stimulate the activity of a common adenyl cyclase system in plasma membranes isolated from rat liver. Their actions are noncompetitive indicating that they act at separate sites in this system. Manganous ion, above 5.0 mm, inhibited selectively the response of the enzyme to glucagon. Inorganic pyrophosphate (1.5 mm), on the other hand, inhibited the response of the enzyme to fluoride but enhanced the response to glucagon. The inhibitory effect of pyrophosphate was noncompetitive with fluoride ion. Highly purified phospholipase A caused a selective loss of the glucagon response and enhanced the stimulatory effect of fluoride ion. Treatment of liver membranes with digitonin also caused a selective loss of hormone response. Inactivation of glucagon response by digitonin was not restricted to the liver membrane adenyl cyclase system; incubation of ghosts of fat cells with digitonin resulted in loss of response of the adenyl cyclase system to glucagon, adrenocorticotropin, secretin, and epinephrine at very low concentrations of the detergent. Digitonin enhanced the response of the fat cell system to fluoride ion. Sodium dodecyl sulfate, over a narrow range of concentrations, inhibited the response of liver membrane adenyl cyclase to glucagon, and enhanced the enzyme's response to fluoride ion. Other detergents caused a parallel loss of the response to both fluoride ion and glucagon. The present findings suggest that glucagon and fluoride ion acti...