Polymerase chain reaction assay of parvovirus B19 DNA in clinical specimens

作者：Jonathan P. Clewley · 发表于：Journal of Clinical Microbiology · 年份：1989 · DOI：10.1128/jcm.27.12.2647-2651.1989 · 被引用次数：121 · 研究领域：Parvovirus B19 Infection Studies、Prenatal Screening and Diagnostics、Dermatological and COVID-19 studies

The polymerase chain reaction (PCR) was used to detect parvovirus B19 DNA in a panel of sera from individuals recently infected with B19, as demonstrated by the presence of anti-B19 immunoglobulin M. Of 95 serum samples, 60 (63%) were found positive by PCR, whereas only 1 was also found positive by dot hybridization. In a control panel of 100 serum samples from individuals with other infections, only 1 serum sample was found positive by PCR, and this was also found positive by dot hybridization. This was probably just a fortuitous discovery of viremia. Placental tissues from women (n = 89) who had proven B19 infections in pregnancy but who gave birth to healthy infants at term were also tested. A total of 74 (83%) were found positive for B19 DNA by PCR. The high rate of detection by PCR probably represents "decay" of viral DNA after the peak of viremia and is not a clinically significant phenomenon.