The relevance of time series in molecular ecology and conservation biology

作者：Jan Christian Habel, Martin Husemann, Aline Finger, Patrick D. Danley, Frank E. Zachos · 发表于：Biological reviews/Biological reviews of the Cambridge Philosophical Society · 年份：2013 · DOI：10.1111/brv.12068 · 被引用次数：96 · 研究领域：Genetic diversity and population structure、Environmental DNA in Biodiversity Studies、Identification and Quantification in Food

The genetic structure of a species is shaped by the interaction of contemporary and historical factors. Analyses of individuals from the same population sampled at different points in time can help to disentangle the effects of current and historical forces and facilitate the understanding of the forces driving the differentiation of populations. The use of such time series allows for the exploration of changes at the population and intraspecific levels over time. Material from museum collections plays a key role in understanding and evaluating observed population structures, especially if large numbers of individuals have been sampled from the same locations at multiple time points. In these cases, changes in population structure can be assessed empirically. The development of new molecular markers relying on short DNA fragments (such as microsatellites or single nucleotide polymorphisms) allows for the analysis of long-preserved and partially degraded samples. Recently developed techniques to construct genome libraries with a reduced complexity and next generation sequencing and their associated analysis pipelines have the potential to facilitate marker development and genotyping in non-model species. In this review, we discuss the problems with sampling and available marker systems for historical specimens and demonstrate that temporal comparative studies are crucial for the estimation of important population genetic parameters and to measure empirically the effects of rec...