Optogenetic astrocyte activation modulates response selectivity of visual cortex neurons in vivo

作者：Gertrudis Perea, Aimei Yang, Edward S. Boyden, Mriganka Sur · 发表于：Nature Communications · 年份：2014 · DOI：10.1038/ncomms4262 · 被引用次数：251 · 研究领域：Photoreceptor and optogenetics research、Neuroscience and Neuropharmacology Research、Neural dynamics and brain function

Astrocytes play important roles in synaptic transmission and plasticity. Despite in vitro evidence, their causal contribution to cortical network activity and sensory information processing in vivo remains unresolved. Here we report that selective photostimulation of astrocytes with channelrhodopsin-2 in primary visual cortex enhances both excitatory and inhibitory synaptic transmission, through the activation of type 1a metabotropic glutamate receptors. Photostimulation of astrocytes in vivo increases the spontaneous firing of parvalbumin-positive (PV+) inhibitory neurons, while excitatory and somatostatin-positive (SOM+) neurons show either an increase or decrease in their activity. Moreover, PV+ neurons show increased baseline visual responses and reduced orientation selectivity to visual stimuli, whereas excitatory and SOM+ neurons show either increased or decreased baseline visual responses together with complementary changes in orientation selectivity. Therefore, astrocyte activation, through the dual control of excitatory and inhibitory drive, influences neuronal integrative features critical for sensory information processing. Astrocytes regulate activity within neuronal networks. Here, the authors use photostimulation to activate astrocytes in the mouse visual cortex, and find that this increases excitatory and inhibitory neuronal synaptic transmission via activation of type 1a metabotropic glutamate receptors.