The tamA gene of Aspergillus nidulans contains a putative zinc cluster motif which is not required for gene function

作者：Meryl A. Davis, Anna J. Small, Sophie Kourambas, Michael J. Hynes · 发表于：Journal of Bacteriology · 年份：1996 · DOI：10.1128/jb.178.11.3406-3409.1996 · 被引用次数：60 · 研究领域：Fungal and yeast genetics research、Polyamine Metabolism and Applications、Metalloenzymes and iron-sulfur proteins

Expression of many nitrogen catabolic enzymes is controlled by nitrogen metabolite repression in Aspergillus nidulans. Although the phenotypes of tamA mutants have implicated this gene in nitrogen regulation, its function is unknown. We have cloned the tamA gene by complementation of a new tamA allele. The tamA sequence shares significant homology with the UGA35/DAL81/DURL gene of Saccharomyces cerevisiae. In vitro mutagenesis of sequences encoding a putative zinc cluster DNA binding domain indicated that this motif is not required for in vivo TamA function.