In vivo cytotoxic T lymphocyte induction with soluble proteins administered in liposomes

作者：Ramyashree Reddy, Fan Zhou, Smita K. Nair, Linke Huang, Barry T. Rouse · 发表于：The Journal of Immunology · 年份：1992 · DOI：10.4049/jimmunol.148.5.1585 · 被引用次数：130 · 研究领域：Immunotherapy and Immune Responses、RNA Interference and Gene Delivery、Monoclonal and Polyclonal Antibodies Research

The in vivo induction of a CTL response usually requires that Ag be endogenously synthesized so that appropriate processing can occur. In most of the few examples where successful CTL induction was reported with proteins and peptides, unacceptable adjuvants or means of Ag formulation were used. In the present report, liposomes were used to incorporate the soluble proteins OVA and beta-galactosidase. This simple and convenient to use approach, which requires minimal amounts of Ag, results in priming for a CD8+ CTL response and the establishment of immunologic memory. The liposome approach may not only prove a convenient means of inducing CTL responses in vivo but may also be useful to study the mechanisms of Ag processing.