Production of branched-chain volatile fatty acids by certain anaerobic bacteria

作者：Milton J. Allison · 发表于：Applied and Environmental Microbiology · 年份：1978 · DOI：10.1128/aem.35.5.872-877.1978 · 被引用次数：189 · 研究领域：Microbial Metabolic Engineering and Bioproduction、Metabolomics and Mass Spectrometry Studies、Probiotics and Fermented Foods

Net production of isobutyric acid, isovaleric acid, and 2-methylbutyric acid by cultures of Bacteroides ruminicola and Megasphaera elsdenii on media that contained Trypticase or casein hydrolysate continued (up to 5 days) after growth had ceased. Only trace quantities of these acids were produced in a medium that contained a mixture of amino acids that did not include the branched-chain amino acids. M. elsdenii produced increased quantities of the branched-chain fatty acids in a medium that contained Trypticase when glucose was reduced or eliminated from the culture medium. However, B. ruminicola produced increased quantities of branched-chain fatty acids and of phenylacetic acid from Trypticase when glucose was supplied at 3 mg/ml rather than at 1 mg/ml. Single strains of Streptococcus bovis, Selenomonas ruminantium, Bacteroides amylophilus, and Butyrivibrio fibrisolvens did not produce branched-chain fatty acids.