A Monoclonal IgM With Antibodylike Specificity for Phospholipids in a Patient With Lymphoma

作者：Michael Robert Cooper, H. J. Cohen, Carolyn C. Huntley, B. Moseley Waite, L. Spees, Charles L. Spurr · 发表于：Blood · 年份：1974 · DOI：10.1182/blood.v43.4.493.493 · 被引用次数：40 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Glycosylation and Glycoproteins Research、Chronic Lymphocytic Leukemia Research

Abstract Both murine and human myeloma proteins have been shown to have antibodylike activity for serum lipoproteins. We studied a 58-yr-old woman (M.L.) with a lymphoma and a VDRL titer of 1:4096 and a nonreactive FTA-ABS. Serum electrophoresis revealed an M-component which was shown to be IgM-kappa by immunoelectrophoresis and 2-mercaptoethanol treatment of the serum reduced the VDRL titer to 1:64. Immunoelectrophoresis (IEP) of the purified IgM against sonicates of the specific components of the VDRL antigen (cardiolipin, lecithin, cholesterol) produced a precipitin line with both cardiolipin and lecithin. All IgM in the serum could be removed by absorption with cardiolipin, and elution experiments demonstrated the specificity of this reaction. A quantitative precipitin test demonstrated that at the point of equivalence, 0.009 mg of antigen (cardiolipin) reacted with 0.4 mg of IgM (antibody). Additional studies showed that purified IgM (M.L.) gave a positive reaction with nine of 15 phospholipids tested. Similar studies on sera from 50 normal individuals, ten patient’s sera without M-components giving false positive VDRL reactions, and serum from one patient with a congenital absence of IgA and IgG and increased IgM showed no evidence of such a precipitin reaction with any phospholipid tested. Thus, it appeared that the IgM-kappa monoclonal protein behaved like an antibody directed against specific phospholipid antigenic components. The role of lipid antigens in the initia...