Chromosomal transformation of Escherichia coli recD strains with linearized plasmids

作者：Chris B. Russell, David S. Thaler, Frederick W. Dahlquist · 发表于：Journal of Bacteriology · 年份：1989 · DOI：10.1128/jb.171.5.2609-2613.1989 · 被引用次数：168 · 研究领域：Bacterial Genetics and Biotechnology、DNA Repair Mechanisms、CRISPR and Genetic Engineering

Wild-type Escherichia coli are resistant to genetic transformation by purified linear DNA, probably in part because of exonuclease activity. We demonstrate that E. coli containing a recD mutation could be easily transformed by linearized plasmids containing a selectable marker. The marker was transferred to the chromosome by homologous recombination, whereas plasmid markers not in the region of homology were lost.