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Comparison of selective media for primary isolation of Campylobacter fetus subsp. jejuni

作者:Charlotte M. Patton, Scott W. Mitchell, M Pötter, Arnold F. Kaufmann · 发表于:Journal of Clinical Microbiology · 年份:1981 · DOI:10.1128/jcm.13.2.326-330.1981 · 被引用次数:50 · 研究领域:Salmonella and Campylobacter epidemiology、Aquaculture disease management and microbiota、Streptococcal Infections and Treatments

Three selective media, Skirrow, Butzler, and a modification of Butzler medium, were compared for the primary isolation of Campylobacter fetus subsp. jejuni. This organism was isolated from 87 of 347 specimens (72 from 240 dogs rectal swabs and 15 from 107 cats rectal swabs). The positive rate for dogs (30%) was twice as high as that for cats (14%). Skirrow and Butzler media were comparable in their isolation of C. fetus subsp. jejuni. A significantly higher rate of positive results was obtained with modified Butzler medium. The best combination of two media was that of modified Butzler and Skirrow media, which detected 98% of the isolates obtained. The percentage of Campylobacter-positive specimens was increased by 9% by holding primary isolation plates 72 h.