Selective amplification of the cytoplasmic domain of the epidermal growth factor receptor gene in glioblastoma multiforme.

作者：L.T. Malden, Ulrike Novak, Andrew Henry Kaye, Anthony W. Burgess · 发表于：PubMed · 年份：1988 · 被引用次数：158 · 研究领域：Chemical Synthesis and Analysis、HER2/EGFR in Cancer Research、Protein Degradation and Inhibitors

Primary brain tumors of glial origin often overexpress epidermal growth factor receptors (EGF-Rs). This may be associated with amplification of the EGF-R gene. We have examined tissue from 23 glioblastoma multiforme tumors and found amplification and rearrangement of the EGF-R gene in four of these. The cytoplasmic domain of the EGF-R gene was invariably amplified in these four tumors, while the epidermal growth factor binding domain was not uniformly amplified in three of these tumors. Western blot analysis of the EGF-R protein revealed high levels of a truncated EGF-R protein in two of the four tumors with EGF-R gene amplification.