Participation of a trisaccharide-lipid in glycosylation of oviduct membrane glycoproteins

作者：W W Chen, William J. Lennarz · 发表于：Journal of Biological Chemistry · 年份：1976 · DOI：10.1016/s0021-9258(19)57006-5 · 被引用次数：61 · 研究领域：Glycosylation and Glycoproteins Research、Carbohydrate Chemistry and Synthesis、Lipid Membrane Structure and Behavior

Preincubation of a hen oviduct membrane preparation with UDP-Nactyl[14C]glucosamine and bacitracin, followed by incubation with GDP-mannose, leads to formation of a chloroform/methanol (2/1)-extractable glycolipid. Treatment of the lipid with mild acid results in the release of a trisaccharide shown to have the structure beta-mannosyl-N-acetylglucosamineyl-N-acetylglucosamine. Incubation of purified trisaccharide-lipid with oviduct membranes in the presence of sodium deoxycholate, Mn2+, and GDP-mannose leads to formation of a labeled glycoprotein with an apparent molecular weight of 25,000...