Structure of the N-asparagine-linked oligosaccharide units of human placental beta-glucocerebrosidase.

作者：Seiichi Takasaki, Gary J. Murray, Francis Scott Furbish, Roscoe O. Brady, J. A. Barranger, Akira Kobata · 发表于：Journal of Biological Chemistry · 年份：1984 · DOI：10.1016/s0021-9258(18)90936-1 · 被引用次数：126 · 研究领域：Carbohydrate Chemistry and Synthesis、Glycosylation and Glycoproteins Research、Pancreatic function and diabetes

The N-asparagine-linked oligosaccharide chains of homogeneous human placental beta-glucocerebrosidase were released by hydrazinolysis, and their structures were analyzed. The sequence of sugars, linkage, and anomeric configuration of the glycosidic bonds were determined. Approximately 20% of the released sugar chain was of the typical high mannose type. The balance was of complex type with biantennary and triantennary structures. As a major component, the following trisialylated oligosaccharide was detected: (formula; see text).