Amino acid sequence of beta-galactosidase. VII. Isolation of the 24 cyanogen bromide peptides

作者：Audrée V. Fowler · 发表于：Journal of Biological Chemistry · 年份：1978 · DOI：10.1016/s0021-9258(17)30401-5 · 被引用次数：33 · 研究领域：Neonatal Health and Biochemistry、Metabolism and Genetic Disorders、Hemoglobin structure and function

All of the 24 cyanogen bromide peptides of beta-galactosidase have been isolated in pure form. Of these 8 ranged in size from 2 to 5 residues and were purified by paper electrophoresis. The 16 large peptides, from 23 to 119 residues, were chromatographed at pH 5.0 on a carboxymethyl-cellulose column in 0.02 M ammonium acetate buffer containing 8 M urea. A number of peptides were obtained in pure from following Sephadex G-50 or G-75 gel filtration. Others were separated on sulfopropyl-Sephadex or diethyl-(2-hydroxylpropylaminoethyl)-Sephadex. There large peptides were obtained in over 50% yield and several others were obtained in more than 25% yield.