The Essentiality of Histidine in the Catalytic Action of Subtilisin

作者：Elizabeth K. Shaw, John Ruscica · 发表于：Journal of Biological Chemistry · 年份：1968 · DOI：10.1016/s0021-9258(18)97808-7 · 被引用次数：50 · 研究领域：Enzyme Production and Characterization、Glycosylation and Glycoproteins Research、Machine Learning in Bioinformatics

Abstract The inactivation of the bacterial protease, subtilisin BPN', has been achieved with a substrate-like reagent, the bromomethyl ketone derived from benzyloxycarbonyl-l-phenylalanine. Loss of hydrolytic function accompanied the alkylation of N-3 of a single histidine residue. The bacterial enzyme has thus been shown to have a catalytic mechanism similar to that of mammalian pancreatic proteolytic enzymes.