Primary Sequence of U-1 Nuclear Ribonucleic Acid of Novikoff Hepatoma Ascites Cells

作者：Ramachandra Reddy, Tae Suk Ro-Choi, Dale Henning, Harris Busch · 发表于：Journal of Biological Chemistry · 年份：1974 · DOI：10.1016/s0021-9258(19)42183-2 · 被引用次数：178 · 研究领域：RNA Research and Splicing、RNA modifications and cancer、Nitrogen and Sulfur Effects on Brassica

The U-1 RNA is one of the low molecular weight nuclear RNAs of Novikoff hepatoma ascites cells that is specifically localized to the extranucleolar portion of the nucleus. After the RNA was purified by polyacrylamide gel electrophoresis, it was subjected to various enzymatic digestion procedures for determination of its primary nucleotide sequence. The U-1 RNA contains 171 nucleotides. Its 5′ end is an alkali-stable tetranucleotide containing N2,2,7-trimethyl guanylic acid. The primary sequence of U-1 RNA is: (m32,2,7G, Am, Um) [see PDF for sequence]