Detection and Characterization of Novel Rotavirus Strains in the United States

作者：Mohanraj Ramachandran, Jon R. Gentsch, Umesh D. Parashar, Shurui Jin, Patricia Woods, Jennifer L. Holmes, Carl D. Kirkwood, Ruth F. Bishop, Harry B. Greenberg, Shozo Urasawa, Giuseppe Gerna, Barbara S. Coulson, K. Taniguchi, Joseph Bresee, Roger I. Glass, the National Rotavirus Strain Surveillance System Collaborating Laboratories · 发表于：Journal of Clinical Microbiology · 年份：1998 · DOI：10.1128/jcm.36.11.3223-3229.1998 · 被引用次数：175 · 研究领域：Viral gastroenteritis research and epidemiology、Viral Infections and Immunology Research、Animal Virus Infections Studies

We recently established a rotavirus strain surveillance system in the United States to monitor the prevalent G serotypes before and after the anticipated implementation of a vaccination program against rotavirus and to identify the emergence of uncommon strains. In this study, we examined 348 rotavirus strains obtained in 1996 to 1997 from children with diarrhea in 10 U.S. cities. Strains were characterized for P and G types, subgroups, and electropherotypes by using a combination of monoclonal antibody immunoassay, reverse transcription-PCR, and hybridization. The four strains most commonly found worldwide comprised 83% of the isolates (P[8]G1, 66.4%; P[4]G2, 8.3%; P[8]G3, 6.9%; P[8]G4, 1.4%), but 9.2% were unusual strains (P[6]G9, 5.5%; P[8]G9, 1.7%; P[6]G1, 1.4%; and P[4]G1 and P[8]G2, 0. 3% each). Strains not typeable for P or G type accounted for 5.5% of the total, while 2.3% of the strains had more than one G type (mixed infections). All P[6]G9 strains tested had short electropherotypes and subgroup I specificity and were detected in 4 of 10 cities, while P[8]G9 strains had long electropherotypes and subgroup II VP6 antigens. Both sequence analysis of the VP7 open reading frame (about 94 to 95% amino acid identity with the VP7 gene of G9 prototype strain WI61) and binding to a G9-specific monoclonal antibody strongly suggest that U.S. G9 strains belong to serotype G9. The high detection rates of unusual rotaviruses with G9 (7.2%) or P[6] (6.9%) specificity in multiple U...