RNA polymerase II transcription factor SIII. I. Identification, purification, and properties.

作者：John Bradsher, Kenneth W. Jackson, Ronald C. Conaway, Joan Weliky Conaway · 发表于：Journal of Biological Chemistry · 年份：1993 · DOI：10.1016/s0021-9258(19)74431-7 · 被引用次数：131 · 研究领域：RNA modifications and cancer、RNA and protein synthesis mechanisms、RNA Research and Splicing

A transcription factor that stimulates synthesis of accurately initiated transcripts by RNA polymerase II has been identified and purified to apparent homogeneity from rat liver nuclear extracts. This factor, which we designate SIII, has a native molecular mass of approximately 140 kDa and is composed of three polypeptides of 110, 18, and 15 kDa. All three polypeptides are required to reconstitute SIII transcriptional activity. SIII appears distinct from previously identified mammalian transcription factors.