The Stimulation of Liver Phosphorylase b by AMP, Fluoride and Sulfate

作者：Willy Stalmans, H G Hers · 发表于：European Journal of Biochemistry · 年份：1975 · DOI：10.1111/j.1432-1033.1975.tb04144.x · 被引用次数：312 · 研究领域：Metabolism and Genetic Disorders、Glycogen Storage Diseases and Myoclonus、Amino Acid Enzymes and Metabolism

1. The activity of liver phosphorylase b from several mammalian species has been studied. The enzyme from rat or mouse has a higher activity than the rabbit enzyme, which is itself more active than pig liver phosphorylase b. 2 The activity of liver phosphorylase b is influenced by anions and by AMP, and these effects are influenced by pH. Fluoride, which is currently added to the assay mixture of phosphorylase a in crude preparations, is about as active as sulfate as a stimulator of phosphorylase b. 3. When assayed at pH 6.1 and in the presence of 0.15 M NaF, the activity of rat liver phosphorylase b reaches 25% of that of the a enzyme; if 1 mM AMP is also present, this value rises to 50%. 4. Methods are described that allow the determination of liver phosphorylase a without interference of b, and the determination of total phosphorylase (a+b) in rat liver.