Effect of exogenous glutathione in extender on the freezability of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa

作者：M.S. Ansari, Bushra Allah Rakha, Nemat Ullah, S. M. H. Andrabi, Sajid Iqbal, M. Khalid, Shamim Akhter · 发表于：RVC Research Online (Royal Veterinary College) · 年份：2010 · 被引用次数：34 · 研究领域：Sperm and Testicular Function、Reproductive Biology and Fertility、Reproductive Physiology in Livestock

The study was designed to investigate the effect of exogenous glutathione supplementation (0.0, 0.5, 1.0, 2.0 and 3.0 mM) in tris-citric acid extender on post-thaw sperm motility, viability, plasma membrane and acrosomal integrity of buffalo (Bubalus bubalis) spermatozoa at 0, 3 and 6 hours after thawing. Glutathione supplementation of the extender upto 2.0 mM concentration increased (P≤0.05) sperm motility, viability, plasma membrane integrity and acrosomal integrity at 0, 3 and 6 hours after thawing in a dose-dependent manner compared to the control. However, glutathione supplementation at higher concentration (3.0 mM) was not beneficial for any of semen quality parameters. It is concluded that glutathione supplementation (up to 2.0 mM) of the extender improves the post-thaw quality of buffalo bull spermatozoa.