Hepatic synthesis and secretory release of plasma alpha-2 (acute phase)-globulin appearing in malignancy.

作者：Edward J. Sarcione · 发表于：PubMed · 年份：1967 · 被引用次数：26 · 研究领域：Erythrocyte Function and Pathophysiology、Hemoglobinopathies and Related Disorders、Neonatal Health and Biochemistry

Summary Isolated perfused rat livers obtained from animals bearing the Walker 256 carcinosarcoma incorporated leucine- 3 H and glucosamine- 14 C into a plasma protein precipitated with a rabbit antiserum specifically directed against rat α 2 (acute phase)-globulin. These data are interpreted to indicate that the liver is a site of synthesis and secretory release of this immunologically distinct plasma component in tumor-bearing rats. These data further indicate that the appearance of this particular α 2 -globulin in the plasma of tumor-bearing animals is a resultant of de novo synthesis by the liver rather than release of preformed and stored globulin. The α 2 (acute phase)-globulin present in liver perfusates from tumor-bearing rats was shown to be immunologically identical with that found in liver perfusates from rats with acute inflammatory reaction to injury. The addition of actinomycin D to perfusions of liver and whole blood from tumor-bearing rats markedly decreased incorporation of both leucine and glucosamine radioactivity into immune precipitates containing plasma α 2 (acute phase)-globulin, but was without demonstrable effect on albumin synthesis. These data are consistent with the conclusion that hepatic synthesis of plasma α 2 (acute phase)-globulin is dependent on DNA-directed messenger RNA synthesis and that the half-life of the specific messenger RNA responsible for its synthesis is substantially different from that responsible for plasma albumin synthesis in ...