The peroxisome proliferator activated receptor regulates malic enzyme gene expression.

作者：Hilde Castelein, T Gulick, Peter E Declercq, G P Mannaerts, D D Moore, Myriam I Baes · 发表于：Journal of Biological Chemistry · 年份：1994 · DOI：10.1016/s0021-9258(18)47083-4 · 被引用次数：155 · 研究领域：Peroxisome Proliferator-Activated Receptors、Adipose Tissue and Metabolism、Metabolism, Diabetes, and Cancer

A new regulatory element for peroxisome proliferator activated receptor (PPAR)/retinoid X receptor (RXR) heterodimers was found in the promoter of the malic enzyme gene. Similar to previously characterized peroxisome proliferator response elements (PPREs), it consists of a direct repeat of sequences related to the half-site consensus AGGTCA with an interspacing of 1 base pair. Specific binding of PPAR/RXR heterodimers to this element was demonstrated. Furthermore, this sequence conferred ciprofibrate responsiveness of a reporter through the homologous malic enzyme or heterologous thymidine kinase promoters. This PPRE presumably mediates the transcriptional effects of peroxisome proliferators on malic enzyme expression. The presence of a PPRE in the promoter of this lipogenic enzyme suggests a broader function for the PPAR in the regulation of lipid metabolism.