hv1 is an evolutionarily conserved H2A variant that is preferentially associated with active genes.

作者：C. David Allis, R Richman, Martin A. Gorovsky, Yvonne Ziegler, B Touchstone, William A. Bradley, Richard G. Cook · 发表于：Journal of Biological Chemistry · 年份：1986 · DOI：10.1016/s0021-9258(17)36034-9 · 被引用次数：103 · 研究领域：Epigenetics and DNA Methylation、Cancer-related gene regulation、RNA modifications and cancer

Polyclonal antibodies to the Tetrahymena macronuclear-specific histone variant h v l cross-react with histone-like molecules from yeast, wheat, and mouse.A novel purification scheme has allowed isolation of sufficient h v l to enable determination of the sequence of 61 amino-terminal residues as well as 2 7 additional internal residues.These data clearly demonstrate that hvl shares a number of conserved sequence elements with the H2A family of histones.Comparison of h v l with H2A.F (=H2A.Z=M1), another evolutionarily conserved H2A variant whose sequence is known, reveals that they share an unblocked amino-terminal alanine (instead of acetylserine) and a distinctive structure in a "variant box" region that distinguishes them from major H2As.In addition, 10 residues have been identified which are identical (or highly similar) in hvl and H2A.F, but are different from residues conserved in the major H2As.Therefore, in many ways h v l resembles chick H2A.F more than the major Tetrahymena H2A.The sites of acetylation of h v l also differ from those of the major Tetrahymena H2As.In spite of their similarities, hvl and H2A.Z differ significantly in their amino termini, and antibodies against h v l do not react with H2A.Z.Interestingly, the nucleolar staining pattern reported with anti-hv1 serum is similar to that reported for an antiserum to another H2A variant, mouse testes-enriched H2A.X.Since both H2A.Z and hvl appear to be enriched in transcriptionally active chromatin, these re...