The In Vitro and In Vivo Effects of a Low-Molecular-Weight Fucoidan on the Osteogenic Capacity of Human Adipose-Derived Stromal Cells

作者：Jéssica Rocha de Souza Pereira, Sophie Portron, Blandine Dizier, Claire Vinatier, Martial Masson, Sophie Sourice, Isabelle Galy–Fauroux, Pierre Corre, Pierre Weiss, Anne‐Marie Fischer, Jérôme Guicheux, Dominique Helley · 发表于：Tissue Engineering Part A · 年份：2013 · DOI：10.1089/ten.tea.2013.0028 · 被引用次数：33 · 研究领域：Seaweed-derived Bioactive Compounds、Bone Tissue Engineering Materials、Mesenchymal stem cell research

Human adipose-derived stromal cells (hASCs) may hold potential for bone tissue engineering. Osteogenic differentiation of these cells is crucial to bone formation. Low-molecular-weight fucoidan (LMWF) is a sulfated polysaccharide that potentiates several growth factors, including pro-angiogenic growth factors. To investigate whether hASC preconditioning with LMWF promoted bone repair, we compared the effects of LMWF and low-molecular-weight heparin on hASC phenotype and osteogenic differentiation. LMWF did not modify the stem-cell phenotype of hASCs but enhanced their osteogenic differentiation (formation of calcium deposits, increased activity and expression of alkaline phosphatase, and increased expression of osteopontin and runt-related transcription factor 2). However, when hASCs were exposed to LMWF before their adhesion to biphasic calcium phosphate particles and implantation in a bone-growth mouse model, no bone formation was apparent after 5 or 8 weeks, probably due to cell death. In conclusion, LMWF may hold promise for enhancing the osteogenic differentiation of hASCs before their implantation. However, concomitant vascularization would be required to enhance bone formation.