Synthesis of a Deoxyribonucleic Acid Sequence Complementary to Ovalbumin Messenger Ribonucleic Acid and Quantification of Ovalbumin Genes

作者：Drew Sullivan, Rafael Palacios, Janet Stavnezer, John M. Taylor, Anthony J. Faras, Mary L. Kiely, Nicholas Summers, J. Michael Bishop, ROBERT T. SCHIMKE · 发表于：Journal of Biological Chemistry · 年份：1973 · DOI：10.1016/s0021-9258(19)43323-1 · 被引用次数：143 · 研究领域：DNA and Nucleic Acid Chemistry、DNA Repair Mechanisms、CRISPR and Genetic Engineering

DNA was synthesized in vitro from a template of purified ovalbumin messenger RNA. The DNA product is complementary to a homogeneous sequence specific for ovalbumin. The amount of ovalbumin sequence in hen oviduct and hen liver DNA was measured by hybridization experiments and found to be 2 copies per diploid genome in both tissues, indicating that there is no selective amplification of ovalbumin genes in the tissue specialized for obalbumin synthesis.