Agar Block Technique for Identification of Mycoplasmas by Use of Fluorescent Antibody

作者：Erik J. Baas, D. E. Jasper · 发表于：Applied Microbiology · 年份：1972 · DOI：10.1128/am.23.6.1097-1100.1972 · 被引用次数：92 · 研究领域：Microbial infections and disease research、Aquaculture disease management and microbiota、Toxoplasma gondii Research Studies

A procedure for staining mycoplasmata colonies directly on agar blocks for examination by fluorescent microscopy is described. Areas of the agar surface appropriate for staining were demarcated by use of Lucite cylinders. Direct fluorescent-antibody staining was superior to indirect staining. The technique was very useful for determining whether cultures were mixed and for identification of mycoplasmas in either pure or mixed cultures.