Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture

作者：David Allen Sack, R. Bradley Sack · 发表于：Infection and Immunity · 年份：1975 · DOI：10.1128/iai.11.2.334-336.1975 · 被引用次数：430 · 研究领域：Escherichia coli research studies

A rapid, potentially clinically useful test for detection of enterotoxigenic Escherichia coli is described. Whole bacterial cultures of enterotoxigenic E. coli, when briefly exposed to Y1 adrenal cells in tissue miniculture, effect a rounding response in the tissue culture that can be discerned at 18 to 24 h. The tissue culture technique agreed with the rabbit ileal loop in all 58 enterotoxigenic and 52 non-enterotoxigenic E. coli strains tested.