Myosin in Polymorphonuclear Leukocytes

作者：Thomas P. Stossel, Thomas Dean Pollard · 发表于：Journal of Biological Chemistry · 年份：1973 · DOI：10.1016/s0021-9258(19)43226-2 · 被引用次数：142 · 研究领域：Cell Adhesion Molecules Research、Muscle Physiology and Disorders、Signaling Pathways in Disease

Myosin was extracted from guinea pig polymorphonuclear leukocytes (granulocytes) homogenized in either 0.6 M KC1 or 0.34 M sucrose solutions.It was purified by precipitation at low ionic strength, ammonium sulfate fractionation, and gel filtration with buffers containing KI and ATP.Myosin isolated from sucrose extracts of granulocytes consisted of essentially one polypeptide that co-migrated with the heavy chain of skeletal muscle myosin on sodium dodecyl sulfate acrylamide gel electrophoresis.Antibody against this protein was prepared in rabbits and reacted with it to yield one precipitin line by immunodiffusion.Myosin isolated from extracts of cells prepared with 0.6 M KC1 was heteroge-