Activation of murine B cell lymphomas. I. Influence of lipopolysaccharide.

作者：Lewis L. Lanier · 发表于：The Journal of Immunology · 年份：1982 · DOI：10.4049/jimmunol.129.3.1130 · 被引用次数：7 · 研究领域：HER2/EGFR in Cancer Research

Abstract The influence of lipopolysaccharide on pre-B and B lymphoma cell lines was investigated. Tumor cells were examined for alterations in cellular proliferation, Ig secretion, and cell surface phenotype after culture in the presence of 50 jug/ml LPS for 24 and 48 hr. Striking changes in surface differentiation antigen expression on the L10A/2J and NBL-DU-B cell lines were observed. Culture in the presence of LPS consistently resulted in a two and one-half to threefold increase in the amount of surface l-Ad and l-E/Cd determinants on the L10A/2J cell line, without changing the quantity of H-2Kd or H-Dd gene products. LPS activation of NBL cells caused a four- to fivefold increase in the quantity of surface IgM and ThB antigens, without significantly increasing the amounts of the cell surface differentiation antigens or inducing a switch in surface Ig isotype or Ig secretion. This preferential increase in quantity of surface antigen on these lines was not simply related to increase in cell size (surface area) after LPS activation, but rather it reflected actual increase in cell surface density of these determinants. Furthermore, the change appeared unrelated to cell cycle regulation. A detailed kinetic analysis of the activation process is presented. These phenotypic changes were noted over a wide range of LPS concentrations (1 to 100 jug/ml), and occurred within 4 hr after cells were cultured in the presence of LPS. Removal of the LPS resulted in the loss of the phenotypi...