H-2 COMPONENTS AND CELLULAR MEMBRANES

作者：JOY H. OZEE, Donald F. Hoelzl Wallach · 发表于：Transplantation · 年份：1967 · DOI：10.1097/00007890-196707000-00009 · 被引用次数：52 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Lymphoma Diagnosis and Treatment、Glycosylation and Glycoproteins Research

Two isoantigenic variants of an A/Sn X A.SW lymphoma (LNSF) were separated into the following subcellular fractions: soluble material, total subcellular particulates, “microsomes”, plasma membrane and endoplasmic reticulm. The distribution in these fractions of 6 H-2 specificities was studied using monospecific or nearly monospecific antisera. Hemagglutination inhibition tests were used to detect soluble antigens. Absorption of hemagglutinating antibodies was employed to detect H-2 specificities in particulate fractions. All of the H-2 specificities known to be present in the tumors were analyzed with respect to their subcellular distribution. H-2.19, H-2.3 and H-2.8 were found to be represented in the plasma membrane fraction but could not be detected on endoplasmic reticulum. H-2.5 was found to be soluble. Some H-2.19 was also located in the soluble fraction. H-2.4 was present in the microsomes and H-2.23, a specificity possibly synonymous with the abandoned H-2.W, was represented in both plasma membrane and endoplasmic reticulum.