RNA-dependent DNA polymerase activity of RNA tumor virus. VI. Processive mode of action of avian myeloblastosis virus polymerase

作者：Jonathan P. Leis · 发表于：Journal of Virology · 年份：1976 · DOI：10.1128/jvi.19.3.932-939.1976 · 被引用次数：35 · 研究领域：Animal Virus Infections Studies、RNA Research and Splicing、RNA and protein synthesis mechanisms

Purified avian myeloblastosis virus (AMV) polymerase consisting of alpha,beta subunits has been shown to act processively in catalyzing DNA synthesis primed with 34S AMV RNA oligo(dT), poly(A)-poly(dT), and poly(I)-poly(dC). DNA transcripts prepared with 34S AMV RNA-oligo(dT)14 and AMV polymerase (alphabeta) have been shown to have a molecular weight of 1.05 X 10(6), or approximately one-third the size of the 34S RNA genome. Polymerase subunit alpha acts nonprocessively with the above templates.