Development of a Micromethod for Identification of Anaerobic Bacteria

作者：Tracy D. Wilkins, Clay Walker · 发表于：Applied Microbiology · 年份：1975 · DOI：10.1128/am.30.5.825-830.1975 · 被引用次数：29 · 研究领域：Microbial Community Ecology and Physiology

A microprocedure was described for determining the carbohydrate fermentation patterns of 48 anaerobic bacteria at one time in microtiter plates. The cultures were transferred into agar-filled wells of microtiter plates with a replicator inside an anaerobic glove box. Fermentation was measured both with a colorimetric indicator and with a small pH electrode. The method was approximately 97% accurate. It would be most useful for laboratories that need to identify large numbers of anaerobes at one time.