Improved Collagen Extraction by Modified Longin Method

作者：Thomas A. Brown, D.E. Nelson, John S. Vogel, John Southon · 发表于：Radiocarbon · 年份：1988 · DOI：10.1017/s0033822200044118 · 被引用次数：1083 · 研究领域：Cardiac Valve Diseases and Treatments、RNA and protein synthesis mechanisms、Infective Endocarditis Diagnosis and Management

A re-evaluation of the Longin collagen-extraction method shows that a lower reflux temperature reduces degradation of protein (“collagen”) remnants. This allows additional purification through ultrafiltration to isolate the >30kDalton fraction of the reflux product.