Molecular Weight Determination of Sendai and Newcastle Disease Virus RNA

作者：Daniel Kolakofsky, E. Boy de la Tour, Hajo Delius · 发表于：Journal of Virology · 年份：1974 · DOI：10.1128/jvi.13.2.261-268.1974 · 被引用次数：88 · 研究领域：Viral Infections and Vectors

The molecular weights of Sendai and Newcastle disease virus RNA were estimated by sedimentation in sucrose gradients and by length measurements in the electron microscope under both denaturing and nondenaturing conditions. Sedimentation analyses under denaturing conditions yielded molecular weight estimates of 2.3 x 10(6) to 2.6 x 10(6), whereas length measurements yielded estimates of 5.2 x 10(6) to 5.6 x 10(6) for both denatured and nondenatured viral RNA. It would appear that the conditions of denaturation used (99% dimethyl sulfoxide at 26 C, and reaction with 1.1 M formaldehyde for 10 min at 60 C) do not equally denature parainfluenza virus RNA and other RNAs, such as cellular rRNA, 45S rRNA precursor, and R17 RNA.