5-Fluoro-2'-deoxyuridine incorporation in L1210 DNA.

作者：Donald W. Kufe, Pierre P Major, E.Michael Egan, EUGENE LOH · 发表于：Journal of Biological Chemistry · 年份：1981 · DOI：10.1016/s0021-9258(19)52479-6 · 被引用次数：102 · 研究领域：Chemical Reactions and Isotopes、Chemical Reaction Mechanisms、Cancer therapeutics and mechanisms

We have employed cesium sulfate density gradient centrifugation to separate RNA and DNA of L1210 cells labeled with [3H]fluorodeoxyuridine. We have analyzed nucleotide and nucleoside digests of purified DNA from the [3H]fluorodeoxyuridine-labeled cells and demonstrate by reverse phase and anion exchange high pressure liquid chromatography the presence of tritium radioactivity co-migrating with fluorodeoxyuridine 5'-monophosphate or fluorodeoxyuridine. These observations demonstrate the internucleotide incorporation of fluorodeoxyuridine in DNA and suggest a new mechanism of action for this cytotoxic and mutagenic agent.