Mesenchymal cell-based repair of large, full-thickness defects of articular cartilage.

作者：Shigeyuki Wakitani, Tatsuhiko Goto, S J Pineda, Randell G. Young, Joseph M. Mansour, Arnold I. Caplan, Victor M. Goldberg · 发表于：Journal of Bone and Joint Surgery · 年份：1994 · DOI：10.2106/00004623-199404000-00013 · 被引用次数：1619 · 研究领域：Osteoarthritis Treatment and Mechanisms、Mesenchymal stem cell research、Periodontal Regeneration and Treatments

UNLABELLED: Osteochondral progenitor cells were used to repair large, full-thickness defects of the articular cartilage that had been created in the knees of rabbits. Adherent cells from bone marrow, or cells from the periosteum that had been liberated from connective tissue by collagenase digestion, were grown in culture, dispersed in a type-I collagen gel, and transplanted into a large (three-by-six-millimeter), full-thickness (three-millimeter) defect in the weight-bearing surface of the medial femoral condyle. The contralateral knee served as a control: either the defect in that knee was left empty or a cell-free collagen gel was implanted. The periosteal and the bone-marrow-derived cells showed similar patterns of differentiation into articular cartilage and subchondral bone. Specimens of reparative tissue were analyzed with use of a semiquantitative histological grading system and by mechanical testing with employment of a porous indenter to measure the compliance of the tissue at intervals until twenty-four weeks after the operation. There was no apparent difference between the results obtained with the cells from the bone marrow and those from the periosteum. As early as two weeks after transplantation, the autologous osteochondral progenitor cells had uniformly differentiated into chondrocytes throughout the defects. This repair cartilage was subsequently replaced with bone in a proximal-to-distal direction, until, at twenty-four weeks after transplantation, the subc...