Exosomes from human saliva as a source of microRNA biomarkers

作者：A Michael, SD Bajracharya, PST Yuen, Huandi Zhou, RA Star, GG Illei, Ilias Alevizos · 发表于：Oral Diseases · 年份：2009 · DOI：10.1111/j.1601-0825.2009.01604.x · 被引用次数：731 · 研究领域：Salivary Gland Disorders and Functions、Extracellular vesicles in disease、MicroRNA in disease regulation

OBJECTIVE: The aim of this study was to examine the presence of microRNAs (miRNAs) within exosomes isolated from human saliva and to optimize and test methods for successful downstream applications. DESIGN: Exosomes isolated from fresh and frozen glandular and whole human saliva were used as a source of miRNAs. The presence of miRNAs was validated with TaqMan quantitative PCR and miRNA microarrays. RESULTS: We successfully isolated exosomes from human saliva from healthy controls and a patient with Sjögren's syndrome. microRNAs extracted from the exosomal fraction were sufficient for quantitative PCR and microarray profiling. CONCLUSIONS: The isolation of miRNAs from easily and non-invasively obtained salivary exosomes with subsequent characterization of the miRNA expression patterns is promising for the development of future biomarkers of the diagnosis and prognosis of various salivary gland pathologies.